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Lab Grimoire
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Coffee
v1.0

Dot Blot

Changes (4)
  • Grayscale quantification of dot blot images via browser-side canvas processing
  • Manual ROI (region of interest) selection with adjustable threshold
  • Density ratio calculation relative to loading control dot
  • Export results as CSV; no data leaves the browser
v1.0

Flow Cytometry

Changes (4)
  • Initial release — FCS 2.0 / 3.0 / 3.1 parsing, scatter plot, overlay histogram, polygon / rectangle / threshold / quadrant gating
  • FlowJo .wsp workspace gate import, color compensation, hierarchical drill-down analysis
  • Multi-file overlay, per-gate statistics (count, %, mean, median, CV), SVG / PNG / CSV export
  • Linear, log, and biexponential (Logicle-approximation) axis scales, dark mode, full-page view
v1.0

IHC Batch

Changes (4)
  • Batch IHC analysis across multiple images in a single session
  • Per-image H-score, positive %, mean DAB OD; summary statistics table
  • Adjustable DAB threshold applied consistently across all images
  • CSV export of batch results; browser-side processing only
v1.0

IHC Analyzer

Changes (4)
  • Automated DAB + hematoxylin color deconvolution on IHC images
  • H-score and Allred score computation; staining intensity histogram
  • Positive-cell percentage and mean DAB optical density
  • All processing runs in the browser — no image upload to server
v1.0

PCA

Changes (4)
  • Principal Component Analysis with automatic data standardization
  • Interactive score plot (PC1 vs PC2) with sample labels and color groups
  • Scree plot showing variance explained per component
  • Loadings table and biplot overlay; supports paste from Excel / Google Sheets