.breadcrumb[data-astro-cid-3vjikg4u]{font-size:.85em;color:var(--text-muted);margin-bottom:1.5em}.breadcrumb[data-astro-cid-3vjikg4u] a[data-astro-cid-3vjikg4u]{color:var(--text-secondary)}.breadcrumb[data-astro-cid-3vjikg4u] a[data-astro-cid-3vjikg4u]:hover{color:var(--accent-light)}.category-filters[data-astro-cid-3vjikg4u]{display:flex;flex-wrap:wrap;gap:.5em;margin-bottom:2em}.category-pill[data-astro-cid-3vjikg4u]{padding:.4em 1em;border-radius:20px;font-size:.85em;border:1px solid var(--border);background:transparent;color:var(--text-secondary);cursor:pointer;transition:all .2s;font-family:inherit}.category-pill[data-astro-cid-3vjikg4u]:hover{border-color:var(--accent-border-hover);color:var(--text-primary)}.category-pill[data-astro-cid-3vjikg4u].active{background:var(--accent-bg-card);border-color:var(--accent);color:var(--accent-light)}.protocol-grid[data-astro-cid-3vjikg4u]{display:grid;grid-template-columns:repeat(auto-fill,minmax(300px,1fr));gap:1.5em}.protocol-card[data-astro-cid-3vjikg4u]{display:flex;flex-direction:column;gap:.5em;padding:1.5em;border-radius:12px;border:.5px solid var(--border);background:var(--bg-card);text-decoration:none;color:var(--text-primary);box-shadow:var(--card-double-rule),var(--card-shadow-rest);transition:all .25s ease}@supports (backdrop-filter: blur(1px)){.protocol-card[data-astro-cid-3vjikg4u]{backdrop-filter:blur(12px);-webkit-backdrop-filter:blur(12px)}}.protocol-card[data-astro-cid-3vjikg4u]:hover{background:var(--card-glow-hover),var(--bg-card-hover);border-color:var(--border-strong);transform:translateY(-2px);box-shadow:var(--card-double-rule),var(--card-shadow-hover);color:var(--text-primary)}.protocol-card[data-astro-cid-3vjikg4u] h3[data-astro-cid-3vjikg4u]{font-size:1.1em;margin:0}.protocol-card[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u]{font-size:.9em;color:var(--text-secondary);margin:0;line-height:1.5}.protocol-card-category[data-astro-cid-3vjikg4u]{font-size:.75em;color:var(--accent-light);text-transform:uppercase;letter-spacing:1px}.protocol-card-meta[data-astro-cid-3vjikg4u]{display:flex;gap:.75em;margin-top:auto;padding-top:.75em;font-size:.8em;color:var(--text-muted)}.protocol-card-difficulty[data-astro-cid-3vjikg4u]{padding:.15em .6em;border-radius:4px;font-size:.75em;font-weight:600}.protocol-card-difficulty[data-astro-cid-3vjikg4u].beginner{background:var(--success-bg);color:var(--bio-green)}.protocol-card-difficulty[data-astro-cid-3vjikg4u].intermediate{background:var(--warning-bg);color:var(--warning-color)}.protocol-card-difficulty[data-astro-cid-3vjikg4u].advanced{background:var(--error-bg);color:var(--error-color)}.protocol-card-duration[data-astro-cid-3vjikg4u],.protocol-card-steps[data-astro-cid-3vjikg4u]{color:var(--text-muted)}.protocol-hero[data-astro-cid-3vjikg4u]{padding:2em;border-radius:16px;border:1px solid var(--accent-border-soft);background:var(--bg-card);margin-bottom:2em}@supports (backdrop-filter: blur(1px)){.protocol-hero[data-astro-cid-3vjikg4u]{backdrop-filter:blur(16px);-webkit-backdrop-filter:blur(16px)}}.protocol-hero[data-astro-cid-3vjikg4u] h1[data-astro-cid-3vjikg4u]{font-size:2em;margin-bottom:.5em}.protocol-hero[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u]{color:var(--text-secondary);font-size:1.05em;margin:0}.protocol-hero-badge[data-astro-cid-3vjikg4u]{display:inline-block;font-size:.75em;letter-spacing:1.5px;text-transform:uppercase;color:var(--accent-light);margin-bottom:.75em}.protocol-meta[data-astro-cid-3vjikg4u]{display:flex;flex-wrap:wrap;gap:.5em;margin-top:1.25em}.meta-pill[data-astro-cid-3vjikg4u]{display:inline-flex;align-items:center;gap:.3em;padding:.3em .8em;border-radius:20px;font-size:.8em;border:1px solid var(--border);color:var(--text-secondary)}.meta-pill[data-astro-cid-3vjikg4u].difficulty-beginner{border-color:var(--success-border);color:var(--bio-green)}.meta-pill[data-astro-cid-3vjikg4u].difficulty-intermediate{border-color:var(--warning-border);color:var(--warning-color)}.meta-pill[data-astro-cid-3vjikg4u].difficulty-advanced{border-color:var(--error-border);color:var(--error-color)}.materials-panel[data-astro-cid-3vjikg4u],.equipment-panel[data-astro-cid-3vjikg4u]{background:var(--bg-secondary);border:1px solid var(--border);border-radius:12px;padding:1.5em;margin-bottom:1.5em}.materials-panel[data-astro-cid-3vjikg4u] h2[data-astro-cid-3vjikg4u],.equipment-panel[data-astro-cid-3vjikg4u] h2[data-astro-cid-3vjikg4u]{font-size:1.1em;margin-bottom:1em;color:var(--text-primary)}.panel-grid[data-astro-cid-3vjikg4u]{display:grid;grid-template-columns:1fr 1fr;gap:.75em}.panel-item[data-astro-cid-3vjikg4u]{font-size:.9em;color:var(--text-secondary);padding:.4em 0}.panel-item[data-astro-cid-3vjikg4u] strong[data-astro-cid-3vjikg4u]{color:var(--text-primary)}.protocol-timeline[data-astro-cid-3vjikg4u]{position:relative;padding-left:2.5em;margin:2em 0}.timeline-line[data-astro-cid-3vjikg4u]{position:absolute;left:.9em;top:0;bottom:0;width:3px;background:linear-gradient(180deg,var(--accent) 0%,var(--bio-green) 100%);border-radius:2px;opacity:.4}.step-node[data-astro-cid-3vjikg4u]{position:absolute;left:-2.5em;top:1.1em;width:22px;height:22px;border-radius:50%;border:3px solid var(--border);background:var(--bg-primary);z-index:1;transition:all .3s}.step-node[data-astro-cid-3vjikg4u].pending{border-color:var(--text-muted)}.step-node[data-astro-cid-3vjikg4u].active{border-color:var(--accent);box-shadow:0 0 8px var(--accent-shadow-strong)}.step-node[data-astro-cid-3vjikg4u].done{border-color:var(--bio-green);background:var(--bio-green)}.step-node[data-astro-cid-3vjikg4u].done:after{content:"✓";position:absolute;top:50%;left:50%;transform:translate(-50%,-50%);font-size:11px;color:#fff;font-weight:700}.step-card[data-astro-cid-3vjikg4u]{position:relative;margin-bottom:1.5em;border-radius:12px;border:1px solid var(--border);background:var(--bg-card);overflow:hidden;transition:all .25s}.step-card[data-astro-cid-3vjikg4u].expanded{border-color:var(--accent-border-soft)}.step-header[data-astro-cid-3vjikg4u]{display:flex;align-items:center;justify-content:space-between;padding:1em 1.25em;cursor:pointer;user-select:none;transition:background .2s}.step-header[data-astro-cid-3vjikg4u]:hover{background:var(--surface-tint)}.step-action[data-astro-cid-3vjikg4u]{font-weight:600;font-size:1em;color:var(--text-primary)}.step-duration[data-astro-cid-3vjikg4u]{font-size:.8em;color:var(--text-muted);white-space:nowrap}.step-body[data-astro-cid-3vjikg4u]{padding:0 1.25em 1.25em;background:var(--bg-secondary);font-size:.95em;line-height:1.7;color:var(--text-secondary)}.step-body[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u]{margin-bottom:.75em}.step-body[data-astro-cid-3vjikg4u] ul[data-astro-cid-3vjikg4u],.step-body[data-astro-cid-3vjikg4u] ol[data-astro-cid-3vjikg4u]{padding-left:1.5em;margin-bottom:.75em}.step-why[data-astro-cid-3vjikg4u]{border-left:3px solid var(--accent);background:var(--accent-bg-soft);padding:.75em 1em;border-radius:0 8px 8px 0;margin:.75em 1.25em;font-size:.9em;color:var(--text-secondary)}.step-why[data-astro-cid-3vjikg4u]:before{content:"💡 Why: ";font-weight:600;color:var(--accent-light)}.step-warning[data-astro-cid-3vjikg4u]{border-left:3px solid var(--warning-color);background:var(--warning-bg);padding:.75em 1em;border-radius:0 8px 8px 0;margin:.75em 1.25em;font-size:.9em;color:var(--text-secondary)}.step-warning[data-astro-cid-3vjikg4u]:before{content:"⚠️ Note: ";font-weight:600;color:var(--warning-color)}.step-warning[data-astro-cid-3vjikg4u].safety{border-left-color:var(--error-color);background:var(--error-bg)}.step-warning[data-astro-cid-3vjikg4u].safety:before{content:"🛑 Safety: ";color:var(--error-color)}.step-animation-badge[data-astro-cid-3vjikg4u]{display:inline-flex;align-items:center;gap:.4em;padding:.35em .8em;border-radius:8px;font-size:.8em;background:var(--accent-bg-soft);border:1px solid var(--accent-border-soft);margin:.5em 1.25em;color:var(--accent-light)}.step-check-btn[data-astro-cid-3vjikg4u]{display:flex;align-items:center;justify-content:center;gap:.5em;width:calc(100% - 2.5em);margin:.75em 1.25em 1.25em;padding:.6em;border-radius:8px;border:1px solid var(--border);background:transparent;color:var(--text-secondary);cursor:pointer;font-family:inherit;font-size:.9em;transition:all .2s;min-height:44px}.step-check-btn[data-astro-cid-3vjikg4u]:hover{border-color:var(--bio-green);color:var(--bio-green);background:var(--bio-green-glow)}.step-check-btn[data-astro-cid-3vjikg4u].checked{border-color:var(--bio-green);color:var(--bio-green);background:var(--success-bg)}.progress-bar-container[data-astro-cid-3vjikg4u]{position:sticky;top:56px;z-index:50;padding:.75em 1em;margin-bottom:1.5em;border-radius:10px;border:1px solid var(--border);background:var(--bg-card);display:flex;align-items:center;gap:1em}@supports (backdrop-filter: blur(1px)){.progress-bar-container[data-astro-cid-3vjikg4u]{backdrop-filter:blur(16px);-webkit-backdrop-filter:blur(16px)}}.progress-bar-text[data-astro-cid-3vjikg4u]{font-size:.85em;font-weight:600;color:var(--text-primary);white-space:nowrap}.progress-bar-track[data-astro-cid-3vjikg4u]{appearance:none;-webkit-appearance:none;flex:1;height:8px;border-radius:4px;background:var(--bg-secondary);overflow:hidden;border:0}.progress-bar-track[data-astro-cid-3vjikg4u]::-webkit-progress-bar{background:var(--bg-secondary);border-radius:4px}.progress-bar-track[data-astro-cid-3vjikg4u]::-webkit-progress-value{border-radius:4px;background:linear-gradient(90deg,var(--accent) 0%,var(--bio-green) 100%)}.progress-bar-track[data-astro-cid-3vjikg4u]::-moz-progress-bar{border-radius:4px;background:linear-gradient(90deg,var(--accent) 0%,var(--bio-green) 100%)}.progress-remaining[data-astro-cid-3vjikg4u]{font-size:.8em;color:var(--text-muted);white-space:nowrap}.version-mismatch-banner[data-astro-cid-3vjikg4u]{padding:.75em 1em;border-radius:8px;border:1px solid var(--warning-border);background:var(--warning-bg);color:var(--warning-color);font-size:.85em;margin-bottom:1em;display:flex;align-items:center;gap:.5em}.related-protocols[data-astro-cid-3vjikg4u]{margin-top:3em;padding-top:2em;border-top:1px solid var(--border)}.related-protocols[data-astro-cid-3vjikg4u] h2[data-astro-cid-3vjikg4u]{font-size:1.3em;margin-bottom:1em}.related-card[data-astro-cid-3vjikg4u]{display:inline-block;padding:1em 1.25em;border-radius:10px;border:1px solid var(--border);background:var(--bg-card);color:var(--text-primary);text-decoration:none;transition:all .2s;margin-right:1em;margin-bottom:.75em}.related-card[data-astro-cid-3vjikg4u]:hover{border-color:var(--accent-border-hover);transform:translateY(-2px);color:var(--text-primary)}.anim-pipette[data-astro-cid-3vjikg4u]{animation:pipetteDrop 2s ease-in-out infinite}.anim-centrifuge[data-astro-cid-3vjikg4u]{animation:centrifugeSpin 1.5s linear infinite}.anim-heat[data-astro-cid-3vjikg4u]{animation:heatPulse 2s ease-in-out infinite}.anim-shake[data-astro-cid-3vjikg4u]{animation:shakeMotion .5s ease-in-out infinite}.anim-incubate[data-astro-cid-3vjikg4u]{animation:incubatePulse 3s ease-in-out infinite}.anim-wash[data-astro-cid-3vjikg4u]{animation:washWave 2s ease-in-out infinite}@keyframes pipetteDrop{0%,to{transform:translateY(0)}30%{transform:translateY(-4px)}50%{transform:translateY(2px)}}@keyframes centrifugeSpin{0%{transform:rotate(0)}to{transform:rotate(360deg)}}@keyframes heatPulse{0%,to{opacity:1;transform:scale(1)}50%{opacity:.7;transform:scale(1.15)}}@keyframes shakeMotion{0%,to{transform:translate(0)}25%{transform:translate(-3px)}75%{transform:translate(3px)}}@keyframes incubatePulse{0%,to{opacity:.6}50%{opacity:1}}@keyframes washWave{0%,to{transform:translate(0)}25%{transform:translate(3px)}75%{transform:translate(-3px)}}@media(prefers-reduced-motion:reduce){.anim-pipette[data-astro-cid-3vjikg4u],.anim-centrifuge[data-astro-cid-3vjikg4u],.anim-heat[data-astro-cid-3vjikg4u],.anim-shake[data-astro-cid-3vjikg4u],.anim-incubate[data-astro-cid-3vjikg4u],.anim-wash[data-astro-cid-3vjikg4u]{animation:none!important}}@media(max-width:640px){.protocol-grid[data-astro-cid-3vjikg4u]{grid-template-columns:1fr}.protocol-hero[data-astro-cid-3vjikg4u]{padding:1.25em}.protocol-hero[data-astro-cid-3vjikg4u] h1[data-astro-cid-3vjikg4u]{font-size:1.5em}.panel-grid[data-astro-cid-3vjikg4u]{grid-template-columns:1fr}.protocol-timeline[data-astro-cid-3vjikg4u]{padding-left:2em}.step-node[data-astro-cid-3vjikg4u]{left:-2em;width:18px;height:18px}.step-header[data-astro-cid-3vjikg4u]{padding:.75em 1em}.step-check-btn[data-astro-cid-3vjikg4u]{min-height:44px}.progress-bar-container[data-astro-cid-3vjikg4u]{flex-wrap:wrap}.protocol-meta[data-astro-cid-3vjikg4u]{gap:.4em}.category-filters[data-astro-cid-3vjikg4u]{gap:.35em}}.tools-hero[data-astro-cid-3vjikg4u],.protocol-hero[data-astro-cid-3vjikg4u],.materials-panel[data-astro-cid-3vjikg4u],.progress-bar-container[data-astro-cid-3vjikg4u],.step-card[data-astro-cid-3vjikg4u],.related-card[data-astro-cid-3vjikg4u],.protocol-card[data-astro-cid-3vjikg4u],.protocol-empty-state[data-astro-cid-3vjikg4u]{border-radius:1.45rem;border:1px solid var(--input-border);background:radial-gradient(circle at top left,var(--surface-glow),transparent 28%),linear-gradient(180deg,var(--surface-overlay),var(--bg-card-raised));box-shadow:var(--box-shadow)}.tools-hero[data-astro-cid-3vjikg4u],.protocol-hero[data-astro-cid-3vjikg4u],.materials-panel[data-astro-cid-3vjikg4u],.progress-bar-container[data-astro-cid-3vjikg4u],.protocol-empty-state[data-astro-cid-3vjikg4u]{padding:clamp(1.15rem,3vw,1.7rem)}.tools-hero[data-astro-cid-3vjikg4u]{margin-bottom:1.5rem}.tools-hero-badge[data-astro-cid-3vjikg4u]{display:inline-flex;align-items:center;justify-content:center;min-height:40px;padding:.5rem .95rem;border-radius:999px;border:1px solid var(--accent-border-hover);background:var(--surface-glow);font-family:var(--font-mono);font-size:.72rem;letter-spacing:.14em;text-transform:uppercase;color:var(--bio-cyan)}.tools-hero[data-astro-cid-3vjikg4u] h1[data-astro-cid-3vjikg4u],.protocol-hero[data-astro-cid-3vjikg4u] h1[data-astro-cid-3vjikg4u],.materials-panel[data-astro-cid-3vjikg4u] h2[data-astro-cid-3vjikg4u],.related-protocols[data-astro-cid-3vjikg4u] h2[data-astro-cid-3vjikg4u]{font-family:var(--font-display)}.category-filters[data-astro-cid-3vjikg4u]{display:flex;flex-wrap:wrap;gap:.6rem;margin:0 0 1.4rem}.category-pill[data-astro-cid-3vjikg4u]{min-height:42px;padding:.62rem 1rem;border-radius:999px;border:1px solid var(--input-border);background:var(--header-bg);color:var(--text-secondary)}.category-pill[data-astro-cid-3vjikg4u]:hover,.category-pill[data-astro-cid-3vjikg4u].active{border-color:var(--accent-border-hover);background:var(--surface-glow);color:var(--text-primary)}.protocol-grid[data-astro-cid-3vjikg4u]{gap:1rem}.protocol-card[data-astro-cid-3vjikg4u]{display:grid;gap:.8rem;padding:1.2rem;transition:transform .22s ease,border-color .22s ease,background .22s ease}.protocol-card[data-astro-cid-3vjikg4u]:hover,.related-card[data-astro-cid-3vjikg4u]:hover{transform:translateY(-3px);border-color:var(--accent-border-hover);background:linear-gradient(180deg,var(--bg-card-hover),var(--bg-card-raised))}.protocol-card-category[data-astro-cid-3vjikg4u],.protocol-card-duration[data-astro-cid-3vjikg4u],.protocol-card-steps[data-astro-cid-3vjikg4u],.protocol-meta[data-astro-cid-3vjikg4u] span[data-astro-cid-3vjikg4u],.progress-bar-text[data-astro-cid-3vjikg4u]{font-family:var(--font-mono);font-size:.72rem;letter-spacing:.1em;text-transform:uppercase}.protocol-card[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u],.protocol-hero[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u],.materials-panel[data-astro-cid-3vjikg4u] li[data-astro-cid-3vjikg4u],.related-card[data-astro-cid-3vjikg4u] p[data-astro-cid-3vjikg4u],.protocol-empty-state[data-astro-cid-3vjikg4u],.version-mismatch-banner[data-astro-cid-3vjikg4u]{color:var(--text-secondary)}.protocol-card-difficulty[data-astro-cid-3vjikg4u]{border-radius:999px;padding:.38rem .75rem;border:1px solid var(--input-border);background:var(--surface-tint)}.step-card[data-astro-cid-3vjikg4u]{overflow:hidden;border:1px solid var(--input-border);background:linear-gradient(180deg,var(--surface-overlay),var(--bg-card-raised))}.step-number[data-astro-cid-3vjikg4u]{background:var(--surface-glow);border-right:1px solid var(--surface-glow);color:var(--bio-cyan)}.step-tip[data-astro-cid-3vjikg4u],.step-warning[data-astro-cid-3vjikg4u],.version-mismatch-banner[data-astro-cid-3vjikg4u]{border-radius:1rem}.progress-bar-container[data-astro-cid-3vjikg4u]{top:72px}.progress-bar-track[data-astro-cid-3vjikg4u]{background:var(--surface-tint-hover)}.related-card[data-astro-cid-3vjikg4u]{padding:1rem 1.15rem}.protocol-empty-state[data-astro-cid-3vjikg4u]{display:grid;place-items:center;text-align:center;min-height:180px}